Sequencing technologies

This unit will cover the theory behind the commonly used sequencing platforms.  You will gain a detailed understanding of how sequence data is generated. You will learn about the many different methodologies for sample and library preparation, and their influence on the data generated. You will gain experience in working with different sequence formats and develop skills to critically assess the quality of next generation sequencing data. You will learn how to perform variant calling for several different classes of genomic variation, and critically evaluate which methods are best suited to particular tasks. You will gain experience in identifying errors in sequence data and integrating your knowledge to allow you to troubleshoot the process of sequence generation.